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Of the nine biopsy samples taken from embryos that produced foals, one was lost when the vial cracked upon thawing. Whole-genome amplification was performed on the remaining eight samples. The amplified DNA was evaluated for sex by PCR for the SRY gene. Because in this test absence of the male SRY signal could occur due to failure of the PCR, the amplified DNA was also evaluated for sex by a duplex PCR for the gene for RNA-binding motif protein, Y-linked (RBMY), which is Y-chromosome specific, and glioma pathogenesis-related protein 1 (GLIPR1), an autosomal gene. The sex was successfully determined in all eight samples (four males and four females), by both of the methods.